| Cat.No.: | PE-3359 |
| Product Name: | Recombinant SIRT2 (50-356) protein |
| Product Overview: | Recombinant SIRT2 (50-356) protein that includes amino acids 50 - 356 of human SIRT2 protein (accession number NM_012237.3) was expressed in E. coli and contains an N-terminal GST-Tag with a molecular weight of 66.5 kDa. The purity of the protein is ≥ 85% by SDS-PAGE. |
| Background: | SIRT2 (Sirtuin 2) is a member of the SIR2 / Sirtuin family of NAD+-dependant deacetylase enzymes. It has multiple targets for deacetylation, including tubulin, p53, HOXA10, FOXO3A, P300 and FOXO1. SIRT2 suppresses adipogenesis by deacetylating FOXO1, promoting the binding of FOXO1 to PPARγ and subsequent repression of PPARγ mediated transcriptional activation. Similar to SIRT1, SIRT2 also deacetylates histone H4 at lysine 16. SIRT2 is present in the cytoplasm during most of the cell cycle except in the G2/M transition and in mitosis, where it is found in the nucleus, in contact with chromatin, and is required for exit from mitosis. |
| Applications: | Useful for histone deacetylase assays and the study of enzyme kinetics, screening inhibitors, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM Tris pH 8.0, 150 mM NaCl, 5% glycerol. |
| Tag: | GST |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0016 | Recombinant Human SIRT1 Lysate | Inquiry |
| EL-0017 | Recombinant Human SIRT2 293 Cell Lysate | Inquiry |
| EL-0018 | Recombinant Human SIRT3 293 Cell Lysate | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0017 | Fluorogenic Sirtuin 5 Substrate | Inquiry |
| SP-0018 | Fluorogenic Sirtuin 6 Substrate | Inquiry |
| Related Gene / Proteins | |||
| Siah2 | SIK1 | SIMC1 | SIN3A |
| SIN3B | SIP1 | Sir2p | SIRT |
| SIRT1 | SIRT2 | SIRT3 | SIRT4 |
| SIRT5 | sirt6 | SIRT7 | SIX3 |
USA
Enter your email here to subscribe.
Follow us on
Easy access to products and services you need from our library via powerful searching tools
