| Cat.No.: | PE-3356 |
| Product Name: | Recombinant SIRT5 protein |
| Product Overview: | Recombinant human SIRT5 was expressed in E.coli as the full length protein (accession number NM_012241.4) with an N-terminal GST-Tag. The molecular weight of the protein is 65.8 kDa. The purity of the protein is > 70% by SDS-PAGE. |
| Background: | SIRT5 (Sirtuin 5) is a member of the SIR2 / Sirtuin family of NAD+-dependant deacetylase enzymes. Sirtuin family enzymes can be found in the cytoplasm, mitochondria or nucleus of a cell and are ubiquitously expressed. SIRT5 has multiple enzymatic functions, such as NAD+-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. SIRT5 catalyzes more efficiently removal of malonyl- and succinyl- and glutaryl- than acetyl-lysine modifications. SIRT5 activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting by mediating desuccinylation and deglutarylation of CPS1. SIRT5 also activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. It can modulate ketogenesis through the desuccinylation and activation of HMGCS2. SIRT5 can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX. |
| Applications: | Suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM Tris pH 8.0, 150 mM NaCl, 5% glycerol. |
| Tag: | GST |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0016 | Recombinant Human SIRT1 Lysate | Inquiry |
| EL-0017 | Recombinant Human SIRT2 293 Cell Lysate | Inquiry |
| EL-0018 | Recombinant Human SIRT3 293 Cell Lysate | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0017 | Fluorogenic Sirtuin 5 Substrate | Inquiry |
| SP-0018 | Fluorogenic Sirtuin 6 Substrate | Inquiry |
| Related Gene / Proteins | |||
| Siah2 | SIK1 | SIMC1 | SIN3A |
| SIN3B | SIP1 | Sir2p | SIRT |
| SIRT1 | SIRT2 | SIRT3 | SIRT4 |
| SIRT5 | sirt6 | SIRT7 | SIX3 |
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