| Cat.No.: | PE-3351 |
| Product Name: | Recombinant ALKBH3 protein |
| Product Overview: | Full length ALKBH3 protein (accession number NP_631917.1) was expressed in E.coli cells with an N-terminal 6xHis tag. The molecular weight of ALKBH3 is 36.9 kDa. |
| Background: | ALKBH3 (AlkB Homolog 3, Alpha-Ketoglutarate Dependent Dioxygenase) is a member of ALKBH protein family. It mediates demethylation of DNA and RNA containing 1-methyladenosine (m1A). It can repair alkylated DNA containing 1-methyladenosine (m1A) and 3-methylcytosine (m3C) by oxidative demethylation. ALKBH3 has a strong preference for single-stranded DNA. It is able to process alkylated m3C within double-stranded regions via its interaction with ASCC3, which promoting DNA unwinding to generate single-stranded substrate needed for ALKBH3. ALKBH3 also acts on RNA via demethylating N(1)-methyladenosine (m1A) RNA, an epigenetic internal modification of messenger RNAs (mRNAs) highly enriched within 5-untranslated regions (UTRs) and in the vicinity of start codons. The oxidative demethylation requires molecular oxygen, alpha-ketoglutarate and iron. |
| Applications: | Suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM Tris pH 8.0, 300 mM NaCl, 10% glycerol, 0.5 mM TCEP. |
| Tag: | His |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0378 | Human ALKBH2 Knockout Cell Line 13bp deletion | Inquiry |
| ◆ Research Kits | ||
| EKIT-0559 | ALKBH5 Chemiluminescent Assay Kit | Inquiry |
| ◆ Antibodies | ||
| EAb-0638 | ALKBH3 Polyclonal Antibody (FITC Conjugated) | Inquiry |
| EAb-0639 | ALKBH3 Polyclonal Antibody (HRP Conjugated) | Inquiry |
| EAb-0640 | ALKBH3 Polyclonal Antibody (Biotin Conjugated) | Inquiry |
| Related Gene / Proteins | |||
| ALKBH1 | ALKBH2 | ALKBH3 | ALKBH4 |
| ALKBH5 | ALKBH6 | ALKBH7 | ALKBH8 |
| ALKBH9 | |||
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