| Cat.No.: | PE-3347 |
| Product Name: | Recombinant ALKBH8 protein |
| Product Overview: | Full length ALKBH8 protein (accession number NP_001287939.1) was expressed in E.coli cells with an N-terminal 6xHis tag. The molecular weight of ALKBH8 is 77.9 kDa. |
| Background: | ALKBH8 (AlkB Homolog 8), also called as ABH8 or TRMT9A, is a S-Adenosyl-L-Methionine-dependent tRNA methyltransferase. It catalyzes the methylation of 5-carboxymethyl uridine to 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its methyltransferase domain. It was reported to have a preference for tRNA(Arg) and tRNA(Glu), and does not bind tRNA(Lys). ALKBH8 can bind tRNA and catalyze the iron and alphaketoglutarate dependent hydroxylation of 5-methylcarboxymethyl uridine at the wobble position of the anticodon loop in tRNA via its dioxygenase domain, giving rise to 5-(S)-methoxycarbonylhydroxymethyluridine. It is also required for normalsurvival after DNA damage. ALKBH8 may inhibit apoptosis and promote cell survival and angiogenesis. |
| Applications: | Suitable for use in enzyme kinetics, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM Tris-HCl pH 8.0, 300 mM NaCl, 10% glycerol, and 0.5 mM TCEP. |
| Tag: | His |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0378 | Human ALKBH2 Knockout Cell Line 13bp deletion | Inquiry |
| ◆ Research Kits | ||
| EKIT-0559 | ALKBH5 Chemiluminescent Assay Kit | Inquiry |
| ◆ Antibodies | ||
| EAb-0638 | ALKBH3 Polyclonal Antibody (FITC Conjugated) | Inquiry |
| EAb-0639 | ALKBH3 Polyclonal Antibody (HRP Conjugated) | Inquiry |
| EAb-0640 | ALKBH3 Polyclonal Antibody (Biotin Conjugated) | Inquiry |
| Related Gene / Proteins | |||
| ALKBH1 | ALKBH2 | ALKBH3 | ALKBH4 |
| ALKBH5 | ALKBH6 | ALKBH7 | ALKBH8 |
| ALKBH9 | |||
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