| Cat.No.: | PE-3320 |
| Product Name: | Recombinant Tet2 (1129-2002) protein |
| Product Overview: | Recombinant TET2 (1129-2002) protein that includes amino acids 1129-2002 of human TET2 proteins (accession number NP_001120680.1) was expressed in Sf9 cells and contains an N-terminal 6×His-Tag and FLAG-Tag with a molecular weight of 102.7 kDa. The purity of the protein is >70% by SDS-PAGE. |
| Background: | TET (Ten Eleven Translocation) family proteins are cytosine oxygenases that catalyze the conversion of 5-methylcytosine (5mC) into 5-hydroxymethylcytosine (5hmC). 5hmC can be further oxidized into 5-formylcytosine (5fC) and 5-carboxylcytosine (5caC) by TET proteins. Methylation at the C5 position of cytosine is an epigenetic modification of the mammalian genome that plays an important role in transcriptional regulation. TET proteins are responsible for initiating the enzymatic deamination process leading to cytosine demethylation.TET2 plays an important role in normal myelopoiesis. Somatic loss-of-function mutations in TET2 gene frequently occur in patients with myeloid malignancies. TET2 loss increased hematopoietic stem cell self-renewal and myeloid transformation. TET2 promotes histone O-GlcNAcylation during gene transcription in embryonic stem cells. In addition, TET2 can be recruited to chromatin by CXXC domain protein IDAX. IDAX downregulates TET2 protein by activating caspase pathway. |
| Applications: | Suitable for use in cytosine oxygenase assays, enzyme kinetics, inhibitor screening, and selectivity profiling. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Species: | Human |
| Formulation: | 25 mM HEPES pH 7.5, 300 mM NaCl, 5% glycerol, 0.04% Triton X-100, 0.2 mM TCEP. |
| Tag: | His & FLAG |
| Expression System: | Baculovirus |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0154 | Recombinant Human TET3 Cell Lysate | Inquiry |
| ◆ Antibodies | ||
| EAb-0176 | TEAD2 Polyclonal Antibody | Inquiry |
| ◆ Cell Lines | ||
| CL-0215 | Human TET1 Knockout Cell Line 1bp insertion | Inquiry |
| CL-0217 | Human TET2 Knockout Cell Line 19bp deletion | Inquiry |
| CL-0218 | Human TET3 Knockout Cell Line 14bp deletion | Inquiry |
| Related Gene / Proteins | |||
| TEAD1 | TEAD2 | telomerase | TEN1 |
| TENR | TERF2IP | TET | TET1 |
| TET2 | TET3 | ||
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