| Cat.No.: | NUC-0074 |
| Product Name: | Recombinant Mononucleosomes H2A Ubiquityl (H2Aub) |
| Description: | This mononucleosome is assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4. Accession numbers: H2A-P04908; H2B-O60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. There is a 5' biotin-TEG group on the DNA. Approximately 50% of the nucleosomes are mono-ubiquitinated on histone H2A lysine 118, while the other 50% are mono-ubiquitinated on both histone H2A lysine 118 and histone H2A lysine 119 (multi-mono-ubiquitinated).Polycomb Repressive Complex 1 (PRC1) is a multi-subunit protein complex that is essential for maintaining epigenetic repression of key developmental loci in embryonic stem cells (ESCs). PRC1 proteins BMI1, RNF2 and others function as a Ubiquitin E3 ligase that specifically mono-ubiquitinates histone H2A (K119) which effects chromatin remodeling in a heritably transmissible manner. BMI1 activity has also been reported as necessary for constitutive heterochromatin formation in mammalian somatic cells. |
| Applications: | Mononucleosomes H2A Ubiquityl, Recombinant Human, Biotinylated Mononucleosomes H2A Ubiquityl, Recombinant Human, Biotinylated are useful as substrates in deubiquitylation (Dub) assays and for inhibitor screening. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 227,000 Da. |
| Species: | Human |
| Formulation: | Mononucleosomes H2A Ubiquityl, Recombinant Human, Biotinylated (27.3 µg protein weight, 50 µg total weight) in 43.9 µl of 25 mM Tris pH 7.5, 150 mM NaCl, 2 mM DTT, 20% glycerol. |
| Tag: | Biotin |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Nucleosomes | ||
| NUC-0007 | Recombinant Tetranucleosomes H3.3 | Inquiry |
| NUC-0008 | Recombinant Mononucleosomes H3.3 | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0009 | Histone H4 peptide (1-21), Biotin-labeled | Inquiry |
| SP-0010 | Histone H4 peptide (1-21) | Inquiry |
| SP-0012 | Histone H3 peptide (21-44), Biotin-labeled | Inquiry |
| Related Gene / Proteins | |||
| HIC1 | HIF1A | HIF1AN | HINFP |
| HIPK1 | HIPK2 | HIRA | Histone |
| Histone H1 | Histone H2A | Histone H2B | Histone H3 |
| Histone H4 | HIV-1 reverse transcriptase | ||
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