| Cat.No.: | NUC-0030 |
| Product Name: | Recombinant Nucleosome, H4R3me1 dNuc |
| Description: | Mononucleosomes assembled from recombinant human histones expressed in E. coli (two each of histones H2A, H2B, H3 and H4; accession numbers: H2A-P04908; H2BO60814; H3.1-P68431; H4-P62805) wrapped by 147 base pairs of 601 positioning sequence DNA. Histone H4 (created by a proprietary synthetic method) contains monomethyl-arginine at position 3. The nucleosome is the basic subunit of chromatin. The 601 sequence is a 147-base pair sequence that has high affinity for histone octamers and is useful for nucleosome assembly and contains a 5’ biotin-TEG group. |
| Applications: | Nucleosome, Recombinant Human, H4R3me1 dNuc, Biotinylated Nucleosome, Recombinant Human, H4R3me1 dNucs are highly purified and are suitable for use as substrates in enzyme screening assays or for effector protein binding experiments. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 199,984.26 Da. |
| Species: | Human |
| Formulation: | Nucleosome, Recombinant Human, H4R3me1 (27.3 μg protein weight, 50 μg total weight) in μl of 10 mM Tris HCl, pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. |
| Tag: | Biotin |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Synthetic Peptides | ||
| SP-0011 | Histone H4 peptide (1-21), Dimethyl R3, Biotin-labeled | Inquiry |
| ◆ Research Kits | ||
| EKIT-0013 | Acetyl Histone H4K12 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0014 | Acetyl Histone H4K12 Quantification Kit (Fluorometric) | Inquiry |
| EKIT-0015 | Acetyl Histone H4K16 Quantification Kit (Colorimetric) | Inquiry |
| EKIT-0016 | Acetyl Histone H4K16 Quantification Kit (Fluorometric) | Inquiry |
| Related Gene / Proteins | |||
| H4H18 | H4K12 | H4K16 | H4K20 |
| H4K31 | H4K44 | H4K5 | H4K59 |
| H4K79 | H4K8 | H4K91 | H4R3 |
| H4S1 | H4S47 | H4T30 | H4T80 |
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