| Cat.No.: | PE-3047 |
| Product Name: | PRMT5 Methyltransferase |
| Description: | Full-length human recombinant PRMT5. PRMT5, a type II arginine methyltransferase, catalyzes the transfer of a methyl group from S-adenosyl-L-methionine (SAM) to an omega-nitrogen of the guanidino function of protein L-arginine residues (omega-monomethylation) and the transfer of a second methyl group to the other omega-nitrogen, yielding symmetric dimethylarginine (sDMA). PRMT5 catalytic activity with certain substrates (e.g. histones H2A, H4) can be weak when PRMT5 is not complexed with MEP50, which may be due to MEP50’s role in substrate binding. However, Reaction Biology has found that PRMT5 alone displays significant activity with GST-GAR. The GAR domain of this substrate (“glycine and arginine rich”) derives from the N-terminus of fibrillarin, a nucleolar protein and RNA methyltransferase that can form a protein complex whose components include PRMT5 and PRMT1. In complex with MEP50, PRMT5 is also a component of multiple macromolecular complexes (e.g. 20S Methylosome, Swi/Snf), is located in both the nucleus and cytoplasm, modifies a variety of substrates and plays roles in chromatin remodeling, RNA processing, and regulation of gene expression, cell growth and differentiation. PRMT5’s pro-proliferative effects and their association with multiple cancers (e.g. lung, breast, ovarian, lymphoid) has led to increased interest in its possible targeting for anti-cancer therapy. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 73.7 kDa |
| Species: | Human |
| Formulation: | Solution of purified recombinant protein in 50 mM Tris/HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM TCEP, 20% (v/v) glycerol. |
| Accession#: | NM_006109 |
| Tag: | Flag |
| Protein Length: | 2-637 |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0027 | UNC0321 (trifluoroacetate salt) | Inquiry |
| BSM-0047 | SGC707 | Inquiry |
| ◆ Antibodies | ||
| EAb-0048 | PRMT6 Polyclonal Antibody | Inquiry |
| EAb-0049 | PRMT7 Polyclonal Antibody | Inquiry |
| EAb-0050 | PRMT1 Polyclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| Pr-SET7 | PRC1 | PRC2 | PRDM1 |
| PRDM10 | PRDM11 | PRDM12 | PRDM13 |
| PRDM14 | PRDM15 | PRDM16 | PRDM17 |
| PRDM2 | PRDM3 | PRDM4 | PRDM5 |
| PRDM6 | PRDM7 | PRDM8 | PRDM9 More > |
| PREP1 | PRIM2 | PRKAA2 | PRKCA |
| PRKCB | PRKCE | PRKDC | PRKRIP1 |
| PRMT | prmt1 | PRMT2 | PRMT3 |
| PRMT5 | prmt6 | PRMT7 | PRMT8 |
| PRMT9 | Progerin | Protamine 2 | Prox1 |
| PRPF31 | PRPF40A | PRSS12 | PRSS55 |
| PRTFDC1 | |||
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