| Cat.No.: | PE-3046 |
| Product Name: | PRMT5(C449S)/MEP50 |
| Description: | Mutant Human recombinant PRMT5 incomplex with human recombinant MEP50, produced by co-expression in an insect cell/baculovirus expression system. PRMT5, a type II argininemethyltransferase, catalyzes the transfer of a methyl group fromS-adenosyl-L-methionine (SAM) to an w-nitrogen, of the guanidino function of protein L-arginine residues (w-monomethylation) and the transfer of a second methyl group to the other w-nitrogen, yielding symmetric dimethylarginine (sDMA). Structuralstudies show the PRMT5/MEP50 complex to consist of four heterodimers. PRMT5catalytic activity is weak when not complexed with MEP50, which may be due toMEP50’s role in substrate binding. A component of multiple macromolecularcomplexes (e.g. 20S Methylosome, Swi/Snf), PRMT5/MEP50 is located in both thenucleus and cytoplasm, modifies a variety of substrates and plays roles inchromatin remodeling, RNA processing, and regulation of gene expression, cellgrowth and differentiation. Its pro-proliferative effects and their associationwith multiple cancers (lung, breast, ovarian, lymphoid) has led to increasinginterest in PRMT5 as a target for anti-cancer therapy. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 73.7/39.9 kDa |
| Species: | Human |
| Formulation: | Solution of purified recombinant protein in 50 mM Tris/HCl, pH 8.0, 110 mM NaCl, 2.2 mM KCl, 3 mM TCEP, 20% (v/v) glycerol. |
| Accession#: | NM_006109, NM_024102 |
| Tag: | Strep/Flag/His |
| Expression System: | Baculovirus |
| Protein Length: | PRMT5 2-637(C-term.) with cysteine substitute for serine-499, MEP50 2-342 (C-term.) |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0027 | UNC0321 (trifluoroacetate salt) | Inquiry |
| BSM-0047 | SGC707 | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0043 | Recombinant Human MECP2 293 Cell Lysate | Inquiry |
| ◆ Antibodies | ||
| EAb-0048 | PRMT6 Polyclonal Antibody | Inquiry |
| EAb-0049 | PRMT7 Polyclonal Antibody | Inquiry |
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