| Cat.No.: | PE-3032 |
| Product Name: | PRDM9 Methyltransferase |
| Description: | Human recombinant PRDM9 expressed in Sf9 insect cells with an N-terminal GST-tag. Catalyzes the transfer of methyl groups from S-adenosyl-L-methionine (SAM) to the epsilon-amino function of protein L-lysine residues, specifically histone H3 lysine-4 (H3K4). Expressed only in meiotic male and female germ cells, PRDM9 is required for fully-functional pairing of homologous chromosomes, double strand break repair, meiotic progression and fertility. PRDM9 binds, via its C-terminal array of zinc fingers (ZnFs), to sequence motifs associated with “hotspots” for homologous recombination (crossovers). Such hotspots are enriched for trimethylated histone H3 lysine-4 (H3K4me3), presumably the result of PRDM9’s H3K4 methyltransferase activity, and part of PRDM9’s role may be to direct meiotic recombination events away from non-PRDM9 H3K4me3 marks, such as those in promoters. PRDM9’s histone methyltransferase activity has received relatively little study, although results with the E. coli-expressed mouse enzyme imply that it is an H3K4 trimethylase that only catalyzes the H3K4me2 to H3K4me3 transition. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 76.3 kDa |
| Species: | Human |
| Formulation: | Solution of purified recombinant protein in 50 mM Tris/HCl pH 7.5, 500 mM NaCl, 1 mM TCEP, 10% glycerol (v/v). |
| Accession#: | NM_020227 |
| Tag: | GST |
| Expression System: | Sf9 Insect Cells |
| Protein Length: | 2-414 |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0027 | UNC0321 (trifluoroacetate salt) | Inquiry |
| BSM-0047 | SGC707 | Inquiry |
| ◆ Antibodies | ||
| EAb-0048 | PRMT6 Polyclonal Antibody | Inquiry |
| EAb-0049 | PRMT7 Polyclonal Antibody | Inquiry |
| EAb-0050 | PRMT1 Polyclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| Pr-SET7 | PRC1 | PRC2 | PRDM1 |
| PRDM10 | PRDM11 | PRDM12 | PRDM13 |
| PRDM14 | PRDM15 | PRDM16 | PRDM17 |
| PRDM2 | PRDM3 | PRDM4 | PRDM5 |
| PRDM6 | PRDM7 | PRDM8 | PRDM9 More > |
| PREP1 | PRIM2 | PRKAA2 | PRKCA |
| PRKCB | PRKCE | PRKDC | PRKRIP1 |
| PRMT | prmt1 | PRMT2 | PRMT3 |
| PRMT5 | prmt6 | PRMT7 | PRMT8 |
| PRMT9 | Progerin | Protamine 2 | Prox1 |
| PRPF31 | PRPF40A | PRSS12 | PRSS55 |
| PRTFDC1 | |||
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