| Cat.No.: | PE-3002 |
| Product Name: | EZH1 Complex |
| Description: | Human recombinant EZH1 (residues 2-747; Genbank Accession # NM_001991; MW = 88 kDa) in complex with human recombinants AEBP2 (2-517; NM_001114176; 57 kDa), EED (2-441; NM_003797; 51 kDa), RbAp48 (2-425; NM_005610; 51 kDa) and SUZ12 (2-739; NM_015355; 86 kDa). Total complex MW is 333.4 kDa. All proteins are full-length (residue 2 through C-terminus) and co-expressed in an insect cell/baculovirus expression system. The EED subunit incorporates an N-terminal Flag-tag and all others include an N-terminal His-tag. Catalyzes the transfer of methyl groups from S-adenosyl-L-methionine (SAM) to the e-amino function of protein L-lysine residues, specifically lysine-27 of histone H3 (H3K27). During development, Polycomb Repressive Complex 2 (PRC2) is the principal methyltransferase responsible for generating trimethylated histone H3 lysine-27 (H3K27me3), an epigenetic mark essential for programmed repression of gene expression. EZH2, which includes a SET methyltransferase domain, functions as the catalytic subunit of PRC2, but can be replaced by the homologous EZH1, which is less catalytically active, although PRC2-Ezh1 has strong repressive activity on transcription independent of its methyltransferase activity9. EZH1 acts to preserve the repressive H3K27me3 mark in embryonic stem cells, complementing the role of EZH28. Multiple recent results suggest important roles for EZH1 in developmental and homeostatic processes, including maintenance of the left-right axis, hair follicle homeostasis and wound repair, skeletal muscle differentiation and adult hematopoiesis. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 333.4 kDa |
| Species: | Human |
| Formulation: | 20 mM Tris-HCl, pH 7.9, 150 mM NaCl, 2 mM MgCl2, 2 mM DTT, 20% glycerol (w/v), 0.01% NP-40. |
| Tag: | Flag & His |
| Expression System: | Baculovirus |
| Protein Length: | EZH1 2-747 , AEBP2 2-517, EED 2-441, RbAp48 2-425, SUZ12 2-739; All full-length |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Bioactive Small Molecules | ||
| BSM-0006 | GSK343 | Inquiry |
| BSM-0059 | 3-Deazaneplanocin A | Inquiry |
| BSM-0060 | 3-Deazaneplanocin A hydrochloride | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0024 | Recombinant Human EZH1 293 Cell Lysate | Inquiry |
| EL-0025 | Recombinant Human EZH2 293 Cell Lysate | Inquiry |
| Related Gene / Proteins | |||
| ezh1 | EZH2 | ||
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