| Cat.No.: | PE-2647 |
| Product Name: | Recombinant human KDM1/LSD1 protein (Active) |
| Background: | Histone demethylase that demethylates both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. |
| Applications: | Functional Studies; SDS-PAGE |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Molecular Weight: | 104 kDa including tags |
| Purity: | >70 % SDS-PAGE. |
| Species: | Human |
| Formulation: | pH: 7.40; Constituents: 0.6% Monobasic dihydrogen sodium phosphate, 0.71% Dibasic monohydrogen sodium phosphate |
| Accession#: | O60341 |
| Alternative Names: | Amine oxidase (flavin containing) domain 2/Amine oxidase, flavin containing, 2/AOF2 |
| Tag: | GST |
| Amino Acid Sequence: | APPEEENESE PEEPSGVEGA AFQSRLPHDR MTSQEAACFP DIISGPQQTQ KVFLFIRNRT LQLWLDNPKI QLTFEATLQQ LEAPYNSDTV LVHRVHSYLE RHGLINFGIY KRIKPLPTKK TGKVIIIGSG VSGLAAARQL QSFGMDVTLL EARDRVGGRV ATFRKGNYVA DLGAMVVTGL GGNPMAVVSK QVNMELAKIK QKCPLYEANG QAVPKEKDEM VEQEFNRLLE ATSYLSHQLD FNVLNNKPVS LGQALEVVIQ LQEKHVKDEQ IEHWKKIVKT QEELKELLNK MVNLKEKIKE LHQQYKEASE VKPPRDITAE FLVKSKHRDL TALCKEYDEL AETQGKLEEK LQELEANPPS DVYLSSRDRQ ILDWHFANLE FANATPLSTL SLKHWDQDDD FEFTGSHLTV RNGYSCVPVA LAEGLDIKLN TAVRQVRYTA SGCEVIAVNT RSTSQTFIYK CDAVLCTLPL GVLKQQPPAV QFVPPLPEWK TSAVQRMGFG NLNKVVLCFD RVFWDPSVNL FGHVGSTTAS RGELFLFWNL YKAPILLALV AGEAAGIMEN ISDDVIVGRC LAILKGIFGS SAVPQPKETV VSRWRADPWA RGSYSYVAAG SSGNDYDLMA QPITPGPSIP GAPQPIPRLF FAGEHTIRNY PATVHGALLS GLREAGRIAD QFLGAMYTLP RQATPGVPAQ QSPSM |
| Sequence Similarities: | Belongs to the flavin monoamine oxidase family.Contains 1 SWIRM domain. |
| Expression System: | E. coli |
| Protein Length: | Protein fragment; 158 to 852 |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Cell Lines | ||
| CL-0015 | Human KDM5B Knockout Cell Line 14bp deletion | Inquiry |
| CL-0087 | Human KDM1A Knockout Cell Line 10bp deletion | Inquiry |
| CL-0088 | Human KDM1B Knockout Cell Line 13bp deletion | Inquiry |
| ◆ Research Kits | ||
| EKIT-0062 | Histone Demethylase LSD1 Activity/Inhibition Assay Kit | Inquiry |
| ◆ Antibodies | ||
| EAb-0071 | KDM1B Polyclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| KDM1 | KDM1A | KDM1B | KDM2 |
| KDM2A | KDM2B | KDM3A | KDM3B |
| KDM4 | KDM4A | KDM4B | KDM4C |
| KDM4D | KDM4E | KDM5 | KDM5A |
| KDM5B | KDM5C | KDM5D | KDM6A More > |
| KDM6B | KDM6C | KDM7 | KDM7A |
| KDM7B | KDM8 | LSD1 | LSD2 |
| LSM2 | LSM3 | LSM5 | LSM6 |
| LSP1 | |||
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