| Cat.No.: | PE-2606 |
| Product Name: | Recombinant Human RIG-I/DDX58 protein |
| Background: | Involved in innate immune defense against viruses. Upon interaction with intracellular dsRNA produced during viral replication, triggers a transduction cascade involving MAVS/IPS1, which results in the activation of NF-kappa-B, IRF3 and IRF7 and the induction of the expression of antiviral cytokines such as IFN-beta and RANTES (CCL5). Detects dsRNA produced from non-self dsDNA by RNA polymerase III, such as Epstein-Barr virus-encoded RNAs (EBERs). Essential for the production of interferons in response to RNA viruses including paramyxoviruses, influenza viruses, Japanese encephalitis virus and HCV. |
| Applications: | SDS-PAGE; Western blot; ELISA |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Appearance: | Liquid |
| Molecular Weight: | 133 kDa including tags |
| Species: | Human |
| Formulation: | pH: 8.00; Constituents: 0.31% Glutathione, 0.79% Tris HCl |
| Accession#: | O95786 |
| Alternative Names: | Ddx58/DDX58_HUMAN/DEAD (Asp Glu Ala Asp) box polypeptide 58 |
| Amino Acid Sequence: | MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGP MEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKL EEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMA GAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDL EDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLEL ALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQI PVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQI LVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSG PLPQVIGLTASVGVGDAKNTDEALDYICKLCASLDASVIATVKHNLEELE QVVYKPQKFFRKVESRISDKFKYIIAQLMRDTESLAKRICKDLENLSQIQ NREFGTQKYEQWIVTVQKACMVFQMPDKDEESRICKALFLYTSHLRKYND ALIISEHARMKDALDYLKDFFSNVRAAGFDEIEQDLTQRFEEKLQELESV SRDPSNENPKLEDLCFILQEEYHLNPETITILFVKTRALVDALKNWIEGN PKLSFLKPGILTGRGKTNQNTGMTLPAQKCILDAFKASGDHNILIATSVA DEGIDIAQCNLVILYEYVGNVIKMIQTRGRGRARGSKCFLLTSNAGVIEK EQINMYKEKMMNDSILRLQTWDEAVFREKILHIQTHEKFIRDSQEKPKPV PDKENKKLLCRKCKALACYTADVRVIEECHYTVLGDAFKECFVSRPHPKP KQFSSFEKRAKIFCARQNCSHDWGIHVKYKTFEIPVIKIESFVVEDIATG VQTLYSKWKDFHFEKIPFDPAEMSK |
| Sequence Similarities: | Belongs to the helicase family.Contains 2 CARD domains.Contains 1 helicase ATP-binding domain.Contains 1 helicase C-terminal domain. |
| Expression System: | Wheat germ |
| Post Translational Modifications: | Isgylated. Conjugated to ubiquitin-like protein ISG15 upon IFN-beta stimulation.Ubiquitinated. Undergoes 'Lys-63'-linked ubiquitination. Lys-172 is the critical site for TRIM25-mediated ubiquitination, for MAVS binding and to induce anti-viral signal transduction. Lys-154, Lys-164 and Lys-172 are critical sites for RNF135-mediated ubiquitination. Deubiquitinated by CYLD, a protease that selectively cleaves 'Lys-63'-linked ubiquitin chains. |
| Protein Length: | Full length protein; 1 to 925 |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Antibodies | ||
| EAb-0055 | Phospho-DDX3X (Thr322) Polyclonal Antibody | Inquiry |
| EAb-0306 | DDX43 Polyclonal Antibody | Inquiry |
| ◆ Extracts & Lysates | ||
| EL-0057 | Recombinant Human DDX20 293 Cell Lysate | Inquiry |
| ◆ Cell Lines | ||
| CL-0119 | Human RING1 Knockout Cell Line 1bp deletion | Inquiry |
| ◆ Synthetic Peptides | ||
| SP-0178 | Human RIG-I/DDX58 peptide | Inquiry |
| Related Gene / Proteins | |||
| DDB2 | Ddit3 | DDX10 | DDX18 |
| DDX20 | DDX28 | DDX3X | DDX41 |
| DDX42 | DDX43 | DDX49 | DDX50 |
| DDX58 | RIF1 | RIG-I | RING1 |
| RING1B | RIZ1 | ||
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