| Cat.No.: | PE-1872 |
| Product Name: | O6-methylguanine-DNA Methyltransferase (human recombinant) |
| Description: | O6-methylguanine-DNA Methyltransferase (MGMT) is a DNA repair enzyme responsible for demethylating O6-methylguanine (O6-mG). The methylation of guanine at O6 to form O6-mG allows base-pairing to thymine rather than cytosine during DNA replication. MGMT prevents mutations by transferring the methyl group from O6-mG to MGMT cysteine 145, restoring guanine. This transfer results in a covalent bond between MGMT cysteine 145 and the methyl group, and so MGMT is a single-turnover, "suicide" enzyme. The assay used to test MGMT is the demethylation of a synthesized, double-stranded, DNA oligonucleotide. Demethylation removes an O6-methyl group to expose a restriction site for the restriction endonuclease PvuII. Lanes A-E show 200 ng of methylated dsDNA treated with increasing amounts of MGMT prior to PvuII digestion. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 26.8 kDa |
| Purity: | ≥50% (by SDS-PAGE) |
| Species: | human |
| Formulation: | 50 mM Tris, pH 8.0, 150 mM sodium chloride, 10% glycerol |
| Accession#: | B4DEE8 |
| Alternative Names: | MGMT |
| Tag: | His |
| Expression System: | E. coli |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Extracts & Lysates | ||
| EL-0142 | Recombinant Human MGMT Cell Lysate | Inquiry |
| ◆ Bioactive Small Molecules | ||
| BSM-0164 | Lomeguatrib | Inquiry |
| ◆ Antibodies | ||
| EAb-0230 | O6-Methylguanine-DNA methyltransferase Polyclonal Antibody | Inquiry |
| EAb-0351 | MGMT Polyclonal Antibody | Inquiry |
| ◆ Cell Lines | ||
| CL-0394 | Human MGMT Knockout Cell Line 2bp deletion | Inquiry |
| Related Gene / Proteins | |||
| MGMT | |||
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