| Cat.No.: | PE-1824 |
| Product Name: | PAD4 (human recombinant; His- and GST-tagged) |
| Description: | Protein arginine deiminases (PADs) are guanidino-modifying enzymes belonging to the amidinotransferase superfamily and are designated PAD1-4 and PAD6. All enzymes are cytosolic except for PAD4 which is localized in the nucleus. PAD4 is a homodimer that functions as a transcriptional coregulator to catalyze the conversion of specific arginine residues to citrulline in a calcium-dependent manner. PAD4 substrates include histones H2A, H3, and H4, whose post-translational modifications play a large role in gene regulation. Benzoylated arginine substrates like N-α-benzoyl-L-arginine ethyl ester (BAEE) have proven to be useful tools for characterization of PAD4, having similar kinetic properties to the natural substrates. PAD4 itself can undergo autocitrullination at several sites, which inhibits its enzymatic activity and may play an important role in regulating citrullination in cells. PAD4 activity is increased in rheumatoid arthritis, producing an abundance of citrulline-containing proteins that can be recognized by autoantibodies, which cause the immune system to attack its own tissues. PAD4 has also been implicated in several other diseases including multiple sclerosis, Alzheimer’s disease, glaucoma, and cancer. |
| Storage: | Store at 4℃ if entire vial will be used within 2-4 weeks. Store at -20℃ or -80℃ for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Molecular Weight: | 103.2 kDa |
| Purity: | ≥85% (by SDS-PAGE) |
| Species: | human |
| Formulation: | 50 mM HEPES, pH 8.0, 300 mM sodium chloride, 1mM DTT, and 10% glycerol |
| Accession#: | Q9UM07 |
| Alternative Names: | PADI4;PADI5;PDI5;Peptidylarginine Deiminase 4;Protein Arginine Deiminase 4 |
| Tag: | His & GST |
| Expression System: | E. coli |
| Protein Length: | 2-663 |
| Warning: | This product is for research use only. Not for use in diagnostic or therapeutic procedures. |
| Product Types | ||
| ◆ Antibodies | ||
| EAb-0059 | PARP Monoclonal Antibody | Inquiry |
| EAb-0061 | PARP Polyclonal Antibody | Inquiry |
| EAb-0062 | PARP (Cleaved) Polyclonal Antibody | Inquiry |
| EAb-0063 | PARP (Cleaved) Polyclonal Antibody | Inquiry |
| EAb-0064 | Paf1 Polyclonal Antibody | Inquiry |
| Related Gene / Proteins | |||
| PABPC1L2A | PABPC5 | PABPN1 | PAD |
| PAD1 | PAD2 | PAD3 | PAD4 |
| PADI1 | PADI2 | PADI3 | PADI4 |
| PADI6 | PAF1 | PAN2 | PAPD1 |
| PAPD4 | PAPD5 | PAPD7 | PAPOLA More > |
| PAPOLB | PARD6A | PARG | PARK2 |
| PARK7 | PARP | PARP1 | PARP10 |
| PARP11 | PARP12 | PARP14 | PARP15 |
| PARP16 | PARP2 | PARP3 | PARP4 |
| PARP6 | PARP7 | PARP8 | PARP9 |
| PAX5 | PAX6 | PAX7 | PAX9 |
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