Common Name :
Urinary Creatinine
Product Overview :
Urinary Creatinine Colorimetric Assay Kit can be used to measure creatinine levels in urine. The assay relies on the Jaffe’ reaction, wherein a yellow/orange color forms when the metabolite is treated with alkaline picrate. The color derived from creatinine is then destroyed at acidic pH. The difference in color intensity measured at 500 nm before and after acidification is proportional to the creatinine concentration. The sample creatinine concentration is determined using a creatinine standard curve.
Description :
Creatine synthesized in kidney, liver, and pancreas is transported in blood to muscle and brain where it is phosphorylated to phosphocreatine. Some free creatine in muscle is converted to creatinine. The amount of creatinine produced is proportional to the individuals muscle mass. In the absence of renal disease, the excretion rate of creatinine in an individual is relatively constant. Thus, urinary creatinine levels may be used as an index of standardization for other tests. Measurement of creatinine clearance is also useful in detecting renal disease and estimating the extent of impairment of renal function.
Storage :
Store the Creatinine Standard at 4°C and the rest of the kit at room temperature (18-26°C).
Kit Components :
Creatinine Standard, 1 vial/3 ml
Creatinine Color Reagent, 1 vial/12 ml
Creatinine Sodium Hydroxide, 1 vial/5 ml
Creatinine Acid Solution, 1 vial/1 ml
Creatinine Sodium Borate, 1 vial/2.5 ml
Creatinine Surfactant, 1 vial/7.5 ml
96-Well Solid Plate(Colorimetric Assay), 1 plate
96-Well Cover Sheet, 1 cover
Detection method :
Colorimetric
Sensitivity :
Under the standardized conditions of the assay described in this booklet, the dynamic range of the kit is 0-15 mg/dl of creatinine.
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