Product Overview :
The kit assay is based on the transfer of radioactively labeled(*) galactose from UDP-galactose to N-acetylD-glucosamine by UDP-galactosyltransferase. The reaction product, a radiolabeled N-acetylD-lactosamine, is then purified by ion-exchange chromatography and quantified.
Description :
UDP-galactosyltransferase (b4GalT1) is a trans-Golgi membrane bound protein that participates in protein processing in the Golgi apparatus. UDP-galactosyltransferase catalyzes the transfer of galactose from UDP-galactose to N-acetyl-D-glucosamine. The exclusive location of UDP-galactosyltransferase in the Golgi apparatus in the vast majority of tissues and cells, makes it a convenient biochemical marker for Golgi membranes.
Applications :
The kit provides a convenient method for quantitative estimation of the UDP-galactosyltransferase activitity in cell or tissue extracts.
Storage :
The kit is shipped on dry ice. The Mini-Columns can be stored at room temperature. The UDP-Gal Solution and Acceptor Solution should be stored at –20 °C. All remaining components can be stored at 2-8 °C.
Kit Components :
Assay Buffer 500 ul
Manganese Chloride Solution 500 ul
Acceptor Solution 500 ul
UDP-Gal Solution 500 ul
Cleanup Resin 60% suspension 50 ml
Mini-Columns 100 each
Elution Solution 100 ml
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