Common Name :
Triglyceride
Product Overview :
Triglyceride Assay Kit provides a simple, reproducible, and sensitive tool for assaying triglycerides in plasma, serum, cell lysates, and tissue homogenate samples. The Triglyceride Colorimetric Assay uses the enzymatic hydrolysis of the triglycerides by lipase to glycerol and free fatty acids. The glycerol released is subsequently measured by a coupled enzymatic reaction system. The glycerol formed in reaction 1 is phosphorylated to glycerol- 3-phosphate in a reaction catalyzed by glycerol kinase. The glycerol-3- phosphate is oxidized by glycerol phosphate oxidase producing dihydroxyacetone phosphate and hydrogen peroxide. Peroxidase catalyzes the redox-coupled reaction of H2O2 with 4-aminoantipyrine (4-AAP) and N-Ethyl-N-(3-sulfopropyl)- m-anisidine (ESPA), producing a brilliant purple color. The absorbance is measured at 540 nm.
Description :
Triglycerides are water-insoluble lipids consisting of three fatty acids esterified to a glycerol backbone. Triglycerides are transported in the blood as core constituents of all lipoproteins, but are major components of triglyceride-rich chylomicrons and very low-density lipoproteins (VLDL). A major source of triglycerides is dietary fat. Dietary fats are hydrolyzed in the gut into free fatty acids and mono- and diglycerides and then transported through the intestinal villi. After absorption through the gut, they are resynthesized into new triglycerides and assembled into chylomicrons. Triglycerides are rapidly hydrolyzed in the capillary beds by lipoprotein lipase, releasing glycerol and free fatty acids, which are absorbed by adipose tissue for storage. When required, lipases hydrolyze triglycerides from adipose tissue into fatty acids and glycerol, which enter the blood stream. Fatty acids are oxidized in the mitochondria and peroxisomes to produce energy. Triglycerides play an important role in metabolism by containing more than twice as much energy as carbohydrates and proteins.
Synonyms :
Triglyceride Colorimetric Assay Kit; TG Assay Kit
Kit Components :
Triglyceride Standard: 1 vial/400 µl
Standard Diluent Assay Reagent (5X): 1 vial/12 ml
Sodium Phosphate Assay Buffer: 1 vial/4 ml
Triglyceride Enzyme Mixture: 1 vial
96-Well Cover Sheet: 1 cover
96-Well Solid Plate (Colorimetric Assay): 1 plate
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