Product Overview :
Oxidants present in the sample oxidize the ferrous ion– chelator complex to ferric ion. The oxidation reaction is prolonged by enhancer molecules, which are abundantly present in the reaction medium. The ferric ion makes a colored complex with chromogen in an acidic medium. The color intensity, which can be measured spectrophotometrically, is related to the total amount of oxidant molecules present in the sample. The assay is calibrated with hydrogen peroxide and the results are expressed in terms of micromolar hydrogen peroxide equivalent per liter (μmol H2O2 Equiv./L).
Storage :
The kit is shipped on wet ice and storage at 2-8 °C is recommended. Stable up to expiry date when stored capped and at 2-8 °C even after start using.
Kit Components :
Reagent 1, 1 x 30 ml: Buffer Solution H2SO4, 25mM pH1.75
Reagent 2, 1 x 8 ml:
Substrate Solution H2SO4, 25mM pH1.75
Ferrous ion, 5 mM
O-dianisidine, 10nM
Standard, 1 x 4 ml: H202, 10 µmol/L
Quality Control - Level 1, 1 x 4 ml: H202, 5 µmol/L
Quality Control - Level 2, 1 x 4 ml: H202, 20 µmol/L
Compatible Sample Types :
Blood serum, heparinised plasma, semen plasma, saliva, urine, cell lysates and tissue homogenates can be used as sample.
Serum samples are stable up to 1 week stored at 4˚C, 6 months at -20˚C and 1 year at -80˚C.
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