Product Overview :
Tn5 DNA Library Prep Kit for Illumina (for 50 ng DNA) is an optimized genomic library construction kit for the Illumina high-throughput sequencing platform, which is suitable for the amount of 50 ng input DNA. Traditional library construction generally includes multi-step operations such as DNA fragmentation, end repair and linker ligation, while our kit adopts Tn5 transposase method which can be completed in one-step reaction. Our kits are easy to operate and greatly reduce library construction time, and significantly reducing the demanded amount of the initial DNA.
Storage :
Store Transposase Mix at 4°C and other components at –20°C.
Kit Components :
Components 24 rxns 96 rxns
Transposase Mix 120 μl 480 μl
2X Tagmentation Buffer 240 μl 960 μl
Amplify Enzyme 24 μl 96 μl
5X Amplify Buffer 240 μl 960 μl
dNTP Mixture 96 μl 384 μl
P5 60 μl 240 μl
P7 60 μl 240 μl
Control DNA 10 μl 10 μl
Note: Control DNA, Escherichia coli Genomic DNA, 50 ng/μl.
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