Product Overview :
Thrombin activity assay kit utilizes the ability of Thrombin to proteolytically cleave a synthetic substrate and release a fluorophore, AMC, which can be easily quantified by fluorescence reader. This assay kit is simple, rapid and can detect Thrombin activity as low as 1 ng in samples.
Applications :
Determine activity of pure Thrombin
Detect the activity of Thrombin in plasma
Storage :
Store kit at -20°C, protected from light. Briefly centrifuge small vials at low speed prior to opening. Read entire protocol before performing the assay.
Thrombin Assay Buffer: Bring to room temperature before use.
Thrombin Enzyme Standard: Prepare a stock solution of Thrombin Enzyme (50 ng/μl) by adding 12 μl of Thrombin Dilution buffer to 4 μl of Thrombin Enzyme Standard. Mix. Aliquot & store at -80°C. Avoid repeated freeze/thaw.
Kit Components :
Thrombin Dilution Buffer 1 ml
Thrombin Assay Buffer 15 ml
Thrombin Enzyme Standard 5 μl
Thrombin Substrate 0.5 ml
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