Product Overview :
Thioredoxin Reductase Colorimetric Assay Kit provides a convenient method for detecting mammalian TrxR activity in tissue homogenates and cell lysates. It is based on the reduction of DTNB (5,5’-dithio-bis(2-dinitrobenzoic acid); Ellman’s reagent) with NADPH to 5-thio-2-nitrobenzoic acid (TNB) which produces a yellow product that is measured at 405-414 nm. The kit includes all reagents needed to assay mammalian TrxR activity. Measurement of TrxR activity by DTNB reduction in the absence and in the presence of aurothiomalate, a specific TrxR inhibitor included in the kit, allows for correction of non-thioredoxin reductase-independent DTNB reduction (i.e., presence of glutathione). The difference between the two results is the DTNB reduction due to TrxR activity.
Storage :
This kit will perform as specified if stored at -20°C.
Kit Components :
TrxR Assay Buffer (10X) 1 vial
Thioredoxin Reductase Control 1 vial
TrxR Inhibitor 2 vials
TrxR DTNB 1 vial
TrxR NADPH 2 vials
TrxR DMSO 1 vial
96-Well Plate (colorimetric assay) 1 plate
96-Well Cover Sheet 1 cover
Sensitivity :
Under the standardized conditions of the assay described in this booklet, the detection range of the assay is from 0.013-0.063 µmol/min/ml of TrxR activity, which is equivalent to an absorbance increase of 0.01-0.05 per minute.
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