Thioredoxin Reductase Activity Assay Kit

Cat.No. :

Kit-0833

Product Overview :

Thioredoxin Reductase Assay Kit provides a convenient colorimetric assay for detecting TrxR activity in various samples. In the assay TrxR catalyzes the reduction of 5, 5’-dithiobis (2-nitrobenzoic) acid (DTNB) with NADPH to 5-thio-2-nitrobenzoic acid (TNB2- ), which generates a strong yellow color (λmax = 412 nm). Since in crude biological samples other enzymes, such as glutathione reductase and glutathione peroxidase can also reduce DTNB, therefore, TrxR specific inhibitor is utilized to determine TrxR specific activity. Two assays are performed. Tthe first measurement is of the total DTNB reduction by the sample, and the second one is the DTNB reduction by the sample in the presence of the TrxR specific inhibitor. The difference between the two results is the DTNB reduction by TrxR.

Storage :

Store the kit at -20°C, protect from light. Warm TrxR Assay Buffer to room temperature (RT) before use. Briefly centrifuge vials prior to opening.

Size :

100 assays

Kit Components :

TrxR Assay Buffer 25 ml
TNB Standard (lyophilized) 1 vial
DTNB (lyophilized) 1 vial
NADPH (lyophilized) 1 vial
TrxR Positive Control 1 vial
TrxR Inhibitor (lyophilized) 1 vial