Superoxide Dismutase (SOD) Activity Colorimetric Assay Kit

Cat.No. :

Kit-2300

Product Overview :

The Colorimetric Superoxide Dismutase (SOD) Assay Kit provides a quick and sensitive method for the measurement of SOD activity in solutions. In the assay, xanthine is converted to superoxide radical ions, uric acid and hydrogen peroxide by xanthine oxidase (XO). Superoxide reacts with SOD560 to generate a product that absorbs around 560 nm. SOD inhibits the reaction of SOD560 with superoxide, thus reduces the absorption at 560 nm. The reduction in the absorption of SOD560 at 560 nm is proportional to SOD activity. The kit can be performed in a convenient 96-well or 384-well microtiter-plate format.

Warning :

Thaw all the kit components at room temperature before starting the experiment.

Size :

200 Tests

Kit Components :

Component A: SOD560, Freeze (< -15 °C), Minimize light exposure, 2 bottles
Component B: 50X Xanthine, Freeze (< -15 °C), Minimize light exposure, 1 vial (100 µL)
Component C: Xanthine Oxidase, Freeze (< -15 °C), Minimize light exposure, 2 vials
Component D: SOD Standard, Freeze (< -15 °C), Minimize light exposure, 1 vial (500 Units)
Component E: Assay Buffer, Freeze (< -15 °C), 1 bottle (20 mL)