Product Overview :
SUCCINATE, or succinic acid, can be found in all plants and animal tissues. It is an intermediate in the citric acid cycle and plays an important role in intracellular energy generation. Succinate is widely used as a flavoring agent in the food, beverage, and pharmaceutical industries due to its low toxicity. The succinate assay provides a simple, one step assay for measuring succinate. In this assay succinate is converted to pyruvate which reacts with specific reagents and dye to form a colored product. The color intensity at 570 nm or fluorescence at Fex/em = 530/585 nm of the reaction product is directly proportional to succinate concentration in the sample.
Applications :
Direct Assays: succinate in food, beverage, agricultural products, and other biological samples.
Notes :
Reagents are for research use only. Briefly centrifuge tubes before opening. Equilibrate all components to room temperature prior assay. Normal precautions for laboratory reagents should be exercised while using the reagents.
Stability :
Shelf life: 6 months after receipt.
Storage :
The kit is shipped on ice. Store all components at -20°C upon receiving.
Kit Components :
Assay Buffer: 10 mL
Enzyme Mix: 120 µL
Cosubstrate: 120 µL
PEP: Dried
Dye Reagent: 120 µL
Standard: 500 µL 20 mM Succinate
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