Product Overview :
The SARS-CoV-2 Spike:ACE2 Inhibitor Screening Assay Kit is designed for screening and profiling inhibitors of this interaction. This kit comes in a convenient 96-well format, with Hislabeled ACE2, purified SARS-CoV-2 Spike protein, HRP-labeled anti-His antibody, and assay buffer for 100 binding reactions. The key to this kit is the high sensitivity of detection of bound Hislabeled ACE2 by HRP-labeled Anti-His. Only a few simple steps on a microtiter plate are required for the assay. First, SARS-CoV-2 Spike is coated on a 96-well plate. Next, ACE2-His is incubated with SARS-CoV-2 Spike on the plate. Finally, the plate is treated with Anti-His-HRP followed by addition of an HRP substrate to produce chemiluminescence, which then can be measured using a chemiluminescence reader.
Description :
The pandemic coronavirus disease 2019 (COVID-19) is caused by Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2). As a first step of the viral replication strategy, the virus attaches to the host cell surface before entering the cell. The Spike protein receptor binding domain (RBD) recognizes and attaches to the Angiotensin-Converting Enzyme 2 (ACE2) receptor found on the surface of type I and II pneumocytes, endothelial cells, and ciliated bronchial epithelial cells. Drugs targeting the interaction between the Spike protein of SARSCoV-2 and ACE2 may offer some protection against the viral infection.
Stability :
Up to 6 months from date of receipt, when stored as recommended.
Kit Components :
SARS-CoV-2 Spike Protein (RBD), mFc Tag, 5 µg, -80°C
ACE2, His-Tag, 5 µg, -80°C
3x Immuno Buffer 1, 50 ml, -20°C
Blocking Buffer 2, 50 ml, +4°C
Anti-His-HRP, 15 µl, -80°C
ELISA ECL substrate A (transparent bottle), 6 ml, Room Temp
ELISA ECL substrate B (brown bottle), 6 ml, Room Temp
96-well white microplate, 1, +4°C
Avoid multiple freeze/thaw cycles!
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