Product Overview :
Rapid, simple & convenient
The assay can detect renin activity as low as 0.75 U/ml.
Description :
Renin (EC 3.4.23.15), also known as an angiotensinogenase, is an enzyme that participates in the renin-angiotensin system (RAS) which mediates extracellular volume (i.e. blood plasma, lymph and interstitial fluid), and arterial vasoconstriction. An over-active renin-angiotensin system leads to vasoconstriction and retention of sodium and water, causing hypertension. Renin inhibitors are widely used for the treatment of hypertension. In Renin Activity Assay Kit, Renin and other proteases hydrolyze the FRET substrate resulting in a product that is detected fluorometrically at Ex/Em = 328/552 nm to give total protease activity. In the presence of a Renin-Specific Inhibitor, hydrolysis of the substrate is only due to the non-specific protease activity. The difference between the total activity and the activity in the presence of Renin Specific Inhibitor, gives the Renin Activity in the sample. This rapid, simple & sensitive kit can detect renin activity as low as 0.75 U/ml.
Applications :
Detects Renin Activity in samples containing renin.
Usage :
For Research Use Only! Not For Use in Humans.
Storage :
Store kit at -20°C, protected from light. Avoid repeated freeze/thaw for all non-buffer components. Briefly centrifuge small vials prior to opening. Read the entire protocol before using the kit.
Kit Components :
• Renin Assay Buffer
• Renin Substrate
• Renin-Specific Inhibitor
• Human Renin (Positive Control) (Lyophilized)
• EDANS Standard (100 µM)
Detection method :
Fluorescence (Ex/Em = 328/552 nm)
Compatible Sample Types :
Recombinant purified protein
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