Product Overview :
Pyrophosphate Assay Kit provides the most robust spectrophotometric method for measuring pyrophosphate. This kit uses our proprietary fluorogenic pyrophosphate sensor that has its fluorescence intensity proportionally dependent upon the concentration of pyrophosphate. Our assay is much easier and more robust than the enzyme-coupling pyrophosphate methods that require at least two enzymes for their pyrophosphate detections. The kit provides all the essential components for assaying pyrophosphate. This kit has been successfully used in high throughput screening (HTS).
Kit Components :
Component A: Assay Buffer, 1 bottle (25 mL), Freeze (<-15 °C)
Component B: PPi Sensor, 1 vial (lyophilized powder), Freeze (<-15 °C), Minimize light exposure
Component C: Pyrophosphate Standard, 1 vial (1 mL, 50 mM), Freeze (<-15 °C), Minimize light exposure
Component D: DMSO, 1 vial (200 µL), Freeze (<-15 °C)