Product Overview :
PRMT5 is a histone methyltransferase that exhibits methylation activity toward H4-R3.
Description :
The PRMT5 Homogeneous Assay Kit is designed to measure the activity of PRMT5 for screening and profiling applications, using AlphaLISA technology. The key to the PRMT5 Homogeneous Assay Kit is a highly specific antibody that recognizes methylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing PRMT5 enzyme is incubated with the biotinylated substrate for one hour. Next, acceptor beads and primary antibody are added, then donor beads, followed by reading the Alpha-counts.
Applications :
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Storage :
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components :
PRMT5/MEP50: 40 µg; -80°C 20 µM S-adenosylmethionine: 250 μl; -80°CPrimary antibody 4: 200 μl; -80°CBiotinylated histone H4 peptide substrate: 2 x 250 rxns; -80°C 4x HMT assay Buffer 2A: 3 ml; -20°C 4x Detection buffer: 2 ml; -20°C
PDF