Product Overview :
PRMT4 (CARM1) is a histone methyltransferase that exhibits methylation activity toward H3-R17.
Description :
The PRMT4 Direct Activity Chemiluminescent Assay kit is designed to measure PRMT4/CARM1 activity activity for screening and profiling applications. The key to the PRMT4 Chemiluminescent Assay Kit is a highly specific antibody that recognizes methylated PRMT4 substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, S-adenosylmethionine is incubated with a sample containing assay buffer and methyltransferase enzyme. Next, primary antibody is added. Finally, the strip plates are treated with an HRP-labeled secondary antibody followed by addition of an HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Applications :
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Storage :
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components :
PRMT4/CARM1 enzyme: 20 µg; -80°C 20 µM S-adenosylmethionine: 250 µl; -80°C Primary antibody 9: 100 µl; -80°C Secondary HRP-labeled antibody 2: 10 µl; -80°C1% BSA: 50 µl; -80°C4x HMT assay buffer 5: 3 ml; -20°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate A (2 components): 6 ml each; +4°C 96-well plate precoated with PRMT4 substrate: 1 plate; +4°C* Decreasing S-adenosylmethionine concentration will make the assay more sensitive to the inhibitors.
PDF