Product Overview :
Simple procedure; takes ~40 minutes
Fast and convenient
Supplied with 100 positive control assays and all other necessary reagents for screening of PLTP inhibitors.
Description :
Phospholipid transfer protein (PLTP) plays an important role in transferring phospholipids between HDL molecules to modulate HDL size and composition and controlling plasma HDL levels. PLTP also transfers phospholipids from lipoproteins to HDL. PLTP plays a key role in reverse cholesterol transport and may promote atherosclerosis. PLTP is, therefore, considered a promising target for pharmacological intervention. PLTP Activity Fluorometric Assay Kit II uses a self-quenched fluorescent phospholipid that can be measured when transferred to an acceptor molecule. The fluorometric intensity is directly proportional to the amount of phospholipid transferred. Rabbit serum is provided as a positive control. This Assay Kit, in addition to measuring activity in serum, is also suitable for testing activity of recombinant protein.
Usage :
This product is furnished for LABORATORY RESEARCH USE ONLY. Not for diagnostic or therapeutic use.
Kit Components :
PLTP Donor Molecule
PLTP Acceptor Molecule
PLTP Assay Buffer (10X)
Positive Control (Rabbit Serum)
Target Species :
Mammalian
Detection method :
Fluorometer
Compatible Sample Types :
Cell Culture Supernatants
Plasma
Serum
Urine
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