Product Overview :
Plant Membrane Protein Extraction Kit is a high yield membrane protein extraction kit, based on chemical rather than detergent. It can extract membrane proteins from various plants, and can be used for crude protein preparation and membrane protein preparation. The extraction process is simple and convenient. The kit contains a mixture of protease inhibitors and phosphatase inhibitor mixture, preventing the protei degradation by protease, providing a guarantee for extracting high quality protein. Extracted Proteins using this kit has natural activity and can be used for a variety of downstream experiments.
Description :
Transmembrane proteins take on various biological functions and play an important role in the occurrence and development of disease. Membrane protein sample preparation needs to take full account of the downstream applications, such as gel analysis and mass spectrometry analysis, so the preparation of membrane protein samples become an insurmountable challenge. Conventional methods of preparing membrane protein samples are solubilization using detergents and surfactants. Detergent treatment will make the membrane protein loss of its natural structure, thus hindering the functional study of membrane proteins.
Storage :
Protease inhibitors store at -20°C;
Protein extracts store at 2-8°C.
Protease inhibitors can also be stored at 2-8 °C before being used. Store at -20 °C after opening.
Synonyms :
Plant membrane protein; extraction; Plant; Membrane Protein Extraction Kit; Membrane Protein
Kit Components :
Extract buffer A: 25ml
Extract buffer B: 250ul
Dilution buffer C: 10ml
Protease inhibitor mixture: 100 ul
Features & Benefits :
1. Easy to use.
2. Contain protein stabilizer, ensuring the stability of extracted protein.
3. Background interference is low when detect protein concentration.
4. The protease inhibitor cocktail consists of 6 independent protease inhibitors; each inhibitor specifically inhibits one or more protease activities. The optimized composition of the mixture allows it to inhibit almost all the important protease activities, including serine proteases, cysteine proteases, aspartic proteases, alanyl-aminopeptidases and so on.
PDF