Product Overview :
Phospholipase A2 Activity Assay Kit provides a quick, sensitive and simple way for measuring PLA2 activity in various biological samples. In this assay, active PLA2 cleaves a synthetic thiophospholipid, producing a lysothiophospholipid which reacts with a fluorogenic probe to produce a highly fluorescent product detectable in the visible range (Ex/Em= 388/513 nm). The assay is simple to perform, high-throughput adaptable and can detect less than 0.1 mU of PLA2 activity.
Applications :
Measurement of phospholipase A2 activity in various tissues/cells, venoms, secretory fluids.
Storage :
Store kit at -20°C, protected from light. Briefly centrifuge small vials prior to opening. Read entire protocol before performing the assay.
Kit Components :
PLA2 Assay Buffer 25 ml
PLA2 Substrate 40 µl
PLA2 Probe 100 µl
Fluorescence Standard 1 vial
Bee Venom Positive Control 1 vial
Compatible Sample Types :
Venoms and secretory fluids (snake venom, bee venom, synovial fluid, etc.)
Soft tissue homogenates (liver, intestine, etc.)
Cultured cells (adherent or suspension cells)
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