Phosphatidylcholine Assay Kit

Cat.No. :

Kit-0662

Product Overview :

Phosphatidylcholine Colorimetric Assay Kit provides a specific, sensitive, and convenient method for quantifying phosphatidylcholine in plasma or serum. In this assay, phosphatidylcholine-specific phospholipase D (PC-Specific PLD) is first used to hydrolyze phosphatidylcholine to choline and phosphatidic acid. The newly formed choline is then used to generate hydrogen peroxide in a reaction catalyzed by choline oxidase. Finally, with peroxidase as a catalyst, hydrogen peroxide reacts with DAOS and 4-aminoantipyrine to generate a blue dye with an optimal absorption at 595 nm.

Storage :

at -20°C. Stability ≥ 1 year

Shipping :

Wet ice

Size :

96 wells

Sensitivity :

Under the standardized conditions of the assay described in this booklet, the dynamic range of the kit is 20-150 mg/dl phosphatidylcholine.