Product Overview :
The PDE3A Assay Kit is designed for identification of PDE3A inhibitors using fluorescence polarization. The assay is based on the binding of a fluorescent nucleotide monophosphate generated by PDE3A to the binding agent. The key to the PDE3A Assay Kit is the specific binding agent. Using this kit, only two simple steps on a microtiter plate are required for PDE3A reactions. First, the fluorescently labeled cAMP is incubated with a sample containing PDE3B for 1 hour. Second, binding agent is added to the reaction mix to produce a change in fluorescent polarization that can then be measured using a fluorescence reader equipped for the measurement of fluorescence polarization.
Applications :
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Storage :
At least 6 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components :
PDE3A recombinant enzyme: >1 µg; -80°C
FAM-Cyclic-3′, 5′-AMP (20 µM): 50 µl; -80°C
PDE assay buffer: 25 ml; -20°C
Binding Agent: 100 µl; +4°C
Binding Agent Diluent: 10 ml; +4°C
Black, low binding, microtiter plate: 1; Room temp.
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