Product Overview :
PARP1 is known to catalyze the NAD-dependent addition of poly (ADP-ribose) to histones.
Description :
The PARP1 Chemiluminescent Activity Assay kit is designed to measure PARP1 activity for screening and profiling applications. The key to the PARP1 Chemiluminescent Activity Assay is the biotinylated substrate. With this kit, only three simple steps are required for PARP1 reactions. First, histone proteins are coated on a 96-well plate. Next, the PARP1 biotinylated substrate is incubated with the assay buffer that contains the PARP1 enzyme. Finally, the plate is treated with streptavidin-HRP followed by addition of the HRP substrate to produce a chemiluminescence that can then be measured using a chemiluminescence reader.
Applications :
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Notes :
DMSO >1%, strong acids or bases, ionic detergents, high salt
Storage :
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Warning :
Avoid freeze/thaw cycles.
Synonyms :
poly (ADP-ribose) polymerase 1; poly(ADP-ribosyl)transferase; ADPRT; PARP1
References :
Brown, J.A., Marala, R.B., J. Pharmacol. Toxicol. Methods. 2002;47:137-41.
Application Reference(s):
1. Dichamanetin inhibits cancer cell growth by affecting ROS-related signaling components through mitochondrial-mediated apoptosis (2013)
2. Structural basis and SAR for G007-LK, a lead stage 1,2,4-triazole based specific tankyrase 1/2 inhibitor (2013)
Kit Components :
PARP1: 5 µg; -80°C
5x histone mixture: 1 ml; -80°C
10x Assay Mixture Containing Biotinylated Substrate: 300 µl; -80°C
10x PARP assay buffer: 1 ml; -20°C
Blocking buffer: 25 ml; +4°C
Activated DNA (5x): 500 µl; -80°C
Streptavidin-HRP: 100 µl; +4°C
HRP chemiluminescent substrate A (translucent bottle): 6 ml; +4°C
HRP chemiluminescent substrate B (brown bottle): 6 ml; +4°C
96-well module plate: 1; Room Temp.
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