Product Overview :
Paraoxonase 1 Activity Assay Kit enables rapid measurement of PON1 activity, utilizing a fluorogenic substrate that is converted into a highly fluorescent product (Ex/Em = 368/460 nm). This ensures dramatically greater sensitivity than UV or colorimetric assays and eliminates the need for dangerous toxic substrates. A selective PON1 inhibitor is provided for verification of PON1 specific activity. The assay is simple to perform, high-throughput adaptable and can detect a minimum of 2.0 μU paraoxonase activity with a sample volume of 5 μl.
Applications :
Rapid assessment of PON1 activity in biological fluids (serum/plasma) or recombinant PON1 preparations.
Storage :
Store kit at -20°C and protect from light. Briefly centrifuge all small vials prior to opening. Allow the Paraoxonase Assay Buffer to warm to room temperature prior to use. Read entire protocol before performing the assay procedure.
• Fluorescence Standard: Reconstitute with 55 µl of DMSO to yield a 5 mM solution. Store at -20°C, stable for 3 freeze/thaw cycles.
• PON1 Inhibitor (2-hydroxyquinoline): Reconstitute with 110 µl of DMSO and vortex to yield a 50 mM stock solution. To prepare a 2mM working solution (10X final concentration), add 40 µl of the 50 mM stock solution to 960 µl of dH2O. The 2 mM working solution should be stored at -20°C and is stable for 3 freeze/thaw cycles.
• PON1 Substrate: Reconstitute with 44 µl of DMSO to obtain a 250X stock solution. Store at -20°C, stable for 3 freeze/thaw cycles.
• Paraoxonase Positive Control: Reconstitute with 110 µl Paraoxonase Assay Buffer and mix thoroughly. Aliquot and store at -80°C, avoid repeated freeze/thaw cycles.
Kit Components :
Paraoxonase Assay Buffer 50 ml NM
Fluorescence Standard 1 vial Yellow
PON1 Inhibitor (2-hydroxyquinoline) 1 vial Clear
PON1 Substrate 1 vial Red
Paraoxonase Positive Control 1 vial Violet
Compatible Sample Types :
• Human or animal plasma and serum
• Heterologously expressed recombinant PON1 preparations
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