Product Overview :
NF-κB (p65) Transcription Factor Assay is a non-radioactive, sensitive method for detecting specific NF-κB (p65) DNA binding activity in nuclear extracts. It replaces the cumbersome radioactive electrophoretic mobility shift assay (EMSA) with an ELISA. This kit is an ideal way to measure NF-κB transcriptional activity downstream of drug treatment or manipulation of cells in vitro or in vivo. NF-κB (p65) Transcription Factor Assay detects human, mouse, and rat NF-κB (p65). It does not cross react with NF-κB (p50).
Storage :
Kit components may be stored at -20°C prior to use. For long-term storage, the Transcription Factor NF-κB (human p65) Positive Control should be thawed on ice, aliquoted at 25 µl/vial, and stored at -80°C.
Kit Components :
Transcription Factor Binding Assay Buffer (4X), 1 vial/3 ml, 4°C
Transcription Factor Reagent A, 1 vial/120 µl, -20°C
Transcription Factor NF-κB (human p65) Positive Control, 1 vial/150 µl, -80°C
Transcription Factor Antibody Binding Buffer (10X), 1 vial/3 ml, 4°C
Transcription Factor NF-κB (p65) Primary Antibody, 1 vial/120 µl, -20°C
Wash Buffer Concentrate (400X), 1 vial/5 ml, RT
Polysorbate 20, 1 vial/3 ml, RT
Transcription Factor NF-κB Competitor dsDNA, 1 vial/120 µl, -20°C
Transcription Factor Goat Anti-Rabbit HRP Conjugate, 1 vial/120 µl, -20°C
Transcription Factor NF-κB 96-Well Strip Plate, 1 plate, 4°C
96-Well Cover Sheet, 1 cover, RT
Transcription Factor Developing Solution, 1 vial/12 ml, 4°C
Transcription Factor Stop Solution, 1 vial/12 ml, RT
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