Product Overview :
Assays of nicotinamide nucleotides are of continual interest in the studies of energy transforming and redox state of cells or tissue. The NADP/NADPH Quantification Kit provides a convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio. The enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NAD;/NADH). There is no need to purify NADP/NADPH from sample mix. The enzyme cycling reaction significantly increases detection sensitivity. Results can be quantified using plate reader at OD450 nm.
Applications :
Convenient tool for sensitive detection of the intracellular nucleotides: NADP, NADPH and their ratio
Kit Components :
NADP/NADPH Extraction Buffer, 50 ml;
NADP Cycling Buffer, 15 ml;
NADP Cycling Enzyme Mix, 0.2 ml;
NADPH Developer, 1 vial;
Stop Solution, 1.2 ml;
NADPH Standard (MW:833.36), 166.7 µg
Target Species :
Mammalian
Detection method :
Absorbance (450 nm)
Features & Benefits :
Simple procedure; takes ~2 hours;
Fast and convenient;
Kit contains the necessary reagents for accurate measurement of NADP and NADPH and their ratio;
Enzymes in the system specifically recognize NADP/NADPH in an enzyme cycling reaction (It does not recognize NAD/NADH)
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