NAD/NADH Assay Kit

Cat.No. :

Kit-2436

Product Overview :

Pyridine nucleotides play an important role in metabolism and, thus, there is continual interest in monitoring their concentration levels. Quantitative determination of NAD+/NADH has applications in research pertaining to energy transformation and redox state of cells or tissue. Simple, direct and automation-ready procedures for measuring NAD+/NADH concentration are very desirable. NAD+/NADH assay kit is based on a lactate dehydrogenase cycling reaction, in which the formed NADH reduces a formazan (MTT) reagent. The intensity of the reduced product color, measured at 565 nm, is proportional to the NAD+/NADH concentration in the sample. This assay is highly specific for NAD+/NADH and with minimal interference (<1%) by NADP+/NADPH. Our assay is a convenient method to measure NAD, NADH and their ratio.

Storage :

The kit is shipped on ice. Store all reagents at -20°C. Shelf life: 6 months after receipt.

Size :

100 tests

Kit Components :

Assay Buffer: 10 mL
Enzyme A: 120 µL
Lactate: 1.5 mL
Enzyme B: 120 µL
MTT Solution: 1.5 mL
NAD Standard: 0.5 mL
NAD(P)/NAD(P)H Extraction Buffers: each 12 mL

Sensitivity :

Detection limit of 0.05 µM and linearity up to 10µM NAD+/NADH in 96-well plate assay.