Monoamine Oxidase A (MAO-A) Inhibitor Screening Kit (Fluorometric)

Cat.No. :

Kit-2361

Product Overview :

Monoamine oxidases (MAO, EC 1.4.3.4) are a family of enzymes that can oxidize a wide variety of endogenous primary amines. Two isoforms, MAO-A and MAO-B, have been identified based on their substrate, inhibitor specificity, and tissue localization. MAO-A can oxidize primary amines such as serotonin and norepinephrine. MAO-A is a mitochondrial-bound enzyme that is ubiquitously expressed throughout the brain and other tissues. It has been implicated in panic, anxiety, and depression. Several MAO-A specific inhibitors such as clorgyline, brofaromine, toloxatone, tetrindole, etc. have been used as antidepressants, but their usage has been limited due to side effects. MAO-A Inhibitor Screening Kit offers a rapid, simple, sensitive, and reliable test suitable for high-throughput screening of MAO-A inhibitors. The assay is based on the fluorometric detection of H2O2, one of the byproducts generated during the oxidative deamination of MAO substrate.

Applications :

Screening/studying/characterizing MAO-A inhibitors

Storage :

-20°C

Shipping :

Gel Pack

Size :

100 assays

Kit Components :

MAO-A Assay Buffer; OxiRed; Probe (in DMSO); MAO-A Enzyme (Lyophilized); MAO-A Substrate (Lyophilized); Developer (Lyophilized); Inhibitor Control (Clorgyline) (Lyophilized)

Detection method :

Fluorescence (Ex/Em = 535/587 nm)

Features & Benefits :

Rapid & sensitive;
High-throughput assay to screen/study/characterize potential inhibitors of monoamine oxidase A;
Includes Inhibitor Control [Clorgyline]