Product Overview :
The MMP-9 Assay Kit provides a convenient assay for high throughput screening of MMP-9 inducers and inhibitors. It detects MMP-9 activity in a variety of biological samples using a 5-FAM/QXL520 fluorescence resonance energy transfer (FRET)5 peptide. In the intact FRET peptide, the fluorescence of 5-FAM is quenched by QXL520. Upon cleavage into two separate fragments by MMP-9 (Scheme 1), the fluorescence of 5-FAM is recovered, and can be monitored at excitation/emission wavelengths = 490 nm/520 nm. With excellent fluorescence quantum yield and longer wavelength, 5-FAM shows less interference from autofluorescence of test compounds and cellular components. Compared to an EDANS/DABCYL FRET substrate, this 5-FAM/QXL520 substrate provides better assay sensitivity. The assays are performed in a convenient 96-well or 384-well microplate format.
Description :
Matrix metalloproteinases (MMPs) belong to a family of secreted or membrane-associated zinc endopeptidases capable of digesting extracellular matrix components.1,2 The importance of MMPs in tumor development and invasion as well as other diseases is well known. MMP-9, (92-kDa gelatinase, collagenase-IV)3,4 is involved in a number of diseases such as cancer, angiogenesis, alopecia, and metastasis. It is proposed as a therapeutic target for these diseases.
Storage :
Store all kit Components at -20centigrade
Protect Components A and B from light and moisture
Components D and E can be stored at 4centigrade for convenience
Kit Components :
Component A: MMP-9 substrate: 5-FAM/QXL520 FRET peptide, Ex/Em=490 nm/520 nm upon cleavage, 60uL
Component B: 5-FAM-Pro-Leu-OH, Fluorescence reference standard Ex/Em=490 nm/520 nm 1 mM, 10 uL
Component C: APMA, 4-aminophenylmercuric acetate 1 M, 20 uL
Component D: Assay buffer, 20 mL
Component E: Stop solution, 10 mL
Features & Benefits :
Convenient Format: All essential assay components are included.
Optimized Performance: Optimal conditions for the detection of MMP-9 activity.
Enhanced Value: Less expensive than the sum of individual components.
High Speed: Minimal hands-on time.
Assured Reliability: Detailed protocol and references are provided.
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