| Cat# | Product Name | Price |
|---|---|---|
| Kit-0243 | Complex IV Human Enzyme Activity Microplate Assay Kit | Inquiry |
| Kit-0244 | Complex IV Human Specific Activity Microplate Assay Kit | Inquiry |
| Kit-0245 | Mit Complex I Activity Assay Kit | Inquiry |
| Kit-0246 | Mit Complex II Activity Assay Kit | Inquiry |
| Kit-0247 | Mit Complex II/III Activity Assay Kit | Inquiry |
| Kit-0248 | Mit Complex IV Activity Assay Kit | Inquiry |
| Kit-0249 | Mitochondrial Complex IV (Mouse) Activity Assay Kit | Inquiry |
| Kit-0250 | Mitochondrial Complex V Activity Assay Kit | Inquiry |
| Kit-2272 | Mitochondrial Complex III Activity Assay Kit | Inquiry |
| Kit-2297 | Mitochondrial Complex I Activity Colorimetric Assay Kit | Inquiry |
Mitochondrial Complex, also known as chimera, is a complex that exists within mitochondria. It consists of several subunits, including NADH-CoQ oxidase, coenzyme Q cytochrome c oxidase, cytochrome c oxidase and ATP synthase complex in the respiratory chain. These subunits work together to guide redox reactions within mitochondria, resulting in the production of cellular energy molecule ATP. Therefore, Mitochondrial Complex is considered an indispensable component in the energy conversion process.
Mitochondrial Complex plays an important role in cellular metabolism, and its biological activity is directly related to the generation of intracellular energy. When the Mitochondrial Complex undergoes changes, a series of diseases may occur, such as metabolic disorders, muscle fiber degeneration, and neurodegenerative diseases.
In order to timely grasp the changes in Mitochondrial Complex, researchers conducted relevant activity measurements.
Mitochondrial Complex activity is mainly measured by measuring its redox ability in cells. This ability can be reflected by detecting the speed at which electrons are transported in the respiratory chain. At present, common methods for measuring Mitochondrial Complex activity include photometry, colorimetry, radioisotope technology, and immunological assay.
Among them, photometry is the most commonly used quantitative analysis method. The main principle is to measure the optical density changes at the absorption wavelength of respiratory chain complexes in mitochondria through their reduction ability. The electron transfer between these cytochromes and coenzymes causes spectral changes, making it possible to measure their activity.
Radioisotope technology is a highly sensitive and accurate measurement method. Using labeled isotopes, the molecules responsible for electron transfer in the respiratory chain will correspond to the emission spectrum. By measuring the content of radioactive isotopes in the sample, accurate and accurate Mitochondrial Complex activity values can be obtained.
Immunoassay is a sophisticated method that can determine how mitochondria respond to regulating energy metabolism by regulating levels. By detecting antibodies with different muscle characteristics in cells, their corresponding Mitochondrial Complex activity can be detected.
This kit can detect enzyme catalyzed NADH oxidation and determine the activity of mitochondrial complex I (NADH dehydrogenase). It has high sensitivity, accuracy, and good repeatability, and can be used for in vitro diagnosis, experimental research, and other biological studies.
This kit is a color chemistry method that can determine the activity of mitochondrial complex II through color detection. This kit has the advantages of fast and simple operation, and can be used to quickly detect physiological and pathological changes related to mitochondrial energy metabolism.
The Mit Complex III detection kit can be used to quantitatively detect the activity of Mit Complex III (mitochondrial respiratory chain complex III) in tissues and cells. This detection method is based on the absorption spectral characteristics of cytochrome C in the cytochrome resonance transmission spectrometer. When the activity of Mit Complex III in the sample is high, a significant increase in spectral characteristic intensity can be observed at the wavelength with maximum absorption capacity.
This detection method has high sensitivity and specificity, and can be used to determine the activity of Mit Complex III in different types of cells and tissues, such as muscle, liver, heart, etc. In addition, this testing kit is also suitable for semi quantitative or rapid screening.
The Mit Complex IV detection kit can be used to quantitatively detect the activity of Mit Complex IV (mitochondrial respiratory chain complex IV) in cells and tissues. This detection method is based on the determination of the catalytic activity of mitochondrial pigment oxidase (COX), which catalyzes the last electron transfer process between cytochrome C and oxygen in the mitochondrial electron transfer chain, thereby generating water and maintaining the normal operation of the respiratory chain.
This detection method can be used to determine the activity level of Mit Complex IV in tissues and cells, for example, to evaluate the functional damage of Mit Complex IV in chronic myopathy, nervous system disease, cardiovascular disease and other diseases. In addition, the detection kit also has high accuracy and specificity, suitable for applications such as high-throughput screening, semi quantitative or quantitative detection.
The Mit Complex V detection kit can be used to quantitatively detect the activity of mitochondrial ATP synthase (complex V). ATP synthase is a major complex in the cellular respiration chain. It catalyzes the combination of ADP and inorganic phosphate in mitochondria to form ATP, and is one of the main sources of ATP production in cells. The detection method is based on the phenomenon of ATPase that mediates enzyme catalysis.
This detection kit has the characteristics of being fast, reliable, and accurate, and can be used to detect the activity level of Mit Complex V in tissues and cells, for evaluation in diseases such as mitochondrial damage or metabolic diseases. In addition, this detection method can also be applied to high-throughput screening, semi quantitative or quantitative testing, and other applications.
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