Product Overview :
Maleimide Assay Kit (Rapid) is a quantitative assay for the quantification of maleimide groups.
Description :
A variety of crosslinking reagents with a maleimide group are widely used for crosslinking proteins to proteins, or proteins to other biomolecules. A key challenge in maleimide crosslinking technology has been the development of reliable and reproducible methods for quantitation the amount of maleimide linked to a single protein or antibody. Maleimides can be directly assayed spectrophotometrically at 302 nm. However, the small extinction coefficient of 620 M-1cm-1 renders this assay insensitive, and the assay is further complicated by the protein absorbance at the same wavelength. There are a few methods available for quantitating the number of maleimide groups by indirect ways. And in many cases, the maleimide ratio is only estimated by experience.
Applications :
Maleimide Assay Kit (Rapid) provides an accurate method to quantify maleimide group by our maleimide sensor Maleimide Blue with the maximum absorbance at ~780nm.
Usage :
For research use only (RUO)
Storage :
Upon receipt, store Maleimide Blue (Component A) the kit at 2-6°C, kept from light and moisture. When stored properly, the kit components should be stable for six months.Note 1: Do not freeze Spin Column (Component C).Note 2: Warm all the components before run the required assays. 50 to 100 μg maleimide-linked antibody or protein sample is needed for determining the amount of maleimide.
Kit Components :
Component A: Maleimide Blue (One vial is for 50 ~100 μg maleimide-linked antibody or protein) 2 vialsComponent B: Assay Buffer 15 mLComponent C: Spin Column 2 columnsComponent D: Washing Tube 2 mLComponent E: Collecting Tube 1.5 mL
Detection method :
Colorimetric
Compatible Sample Types :
Polymers, Proteins
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