Product Overview :
The non-radioactive, colorimetric MDH assay is based on the reduction of the tetrazolium salt MTT in a NADH-coupled enzymatic reaction to a reduced form of MTT which exhibits an absorption maximum at 565 nm. The increase in absorbance at 565 nm is proportional to the enzyme activity.
Description :
MALATE DEHYDROGENASE (MDH) (EC 1.1.1.37) is an enzyme which reversibly catalyzes the oxidation of L-malate to oxaloacetate in the presence of NAD. There are 2 isoforms in eukaryotic cells: MDH1 and MDH2. MDH1 found in the cytoplasm and plays a key part in the malateaspartate shuttle for transporting malate into the mitochondria. MDH2 is a mitochondrial enzyme which participates in the TCA cycle that reversibly converts L-malate into oxaloacetate. Higher MDH activities are found in some neurodegenerative diseases such as Alzheimer"s disease.
Applications :
For quantitative determination of L-malate dehydrogenase enzyme activity and drug effects on malate dehydrogenase metabolism.
Storage :
The kit is shipped on ice. Store all components at -20°C upon receiving. Shelf life: 6 months after receipt.
Kit Components :
Assay Buffer: 10 mL Enzyme A: 120 µLNAD/MTT: 1 mL Enzyme B: 120 µLSubstrate: 600 µL Calibrator: 1.5 mL
Detection method :
OD565nm
Compatible Sample Types :
Biological samples (e.g. plasma, serum, erythrocytes, tissue and culture media).
Features & Benefits :
• Fast and sensitive. Linear detection range (20 μL sample): 0.5 to 65 U/L for 20 min reaction at 37°C.• Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
Sensitivity :
Detection Limit: 0.5 U/L
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