Product Overview :
In Lipoxygenase (LOX) activity assay, lipoxygenase converts the LOX substrate to an intermediate that reacts with the probe generating a fluorescent product. The increase in fluorescent signal can be recorded at Ex/Em 500/536 nm and is directly proportional to LOX activity. The kit includes 5-lipoxygenase enzyme (LOX Enzyme) as a positive control. A lipoxygenase inhibitor that completely inhibits lipoxygenase activity is also included in order to calculate the specific activity of LOX in biological samples. The kit can detect as low as 0.004 mU/mg protein.
Applications :
Measurement of Lipoxygenase activity in cell / tissue lysates and serum using a 96-well plate format.
Storage :
Upon arrival, store the kit at -20°C. Components are stable for at least three months.
• LOX Buffer and LOX Lysis Buffer: Thaw at RT before use. Keep Lysis Buffer on ice.
• Oxidized Probe Standard: Oxidized probe standard is 100X. Aliquot and store at -20°C in the dark.
• LOX Probe and LOX Inhibitor: Aliquot and store at -20°C in the dark.
• LOX Substrate: Store at -20°C in the dark.
• LOX Enzyme: Aliquot and store at -20°C. Avoid repeated freeze thawing of the aliquots.
Note: Store all kit components on ice while performing the assay.
Kit Components :
LOX Assay Buffer; 25 ml
Oxidized Probe Standard (100 μM); 200 µl
LOX Probe; 220 µl
LOX Substrate; 6 µl
LOX Inhibitor; 100 µl
LOX Enzyme; 22 µl
LOX Lysis Buffer; 2 ml
Compatible Sample Types :
Cell lysate (i.e., MCF-7 or other cell types expected to have high lipoxygenase activity)
Biological fluids eg. serum
Recombinant enzyme
Purified Protein
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