Description :
Lactic acid, a major stereoisomer of lactate formed in human intermediary metabolism, has often been used as a physiological indicator for stress. Its determination in serum (normal range in blood 0.5-2.0 mM) is very important in the diagnosis and medical management of various diseases, such as tissue hypoxia, diabetes, circulatory failure and hematological disorders. Unlike a typical lactic acid-LDH assay, this new lactic acid assay kit provides a simple, reliable method for quantifying lactic acid in biological samples such as blood (serum and plasma), culture and fermentation media, etc. In the assay, lactic acid is oxidized by enzyme reactions to yield color product, which can be measured at 570 nm for colorimetric assay, And the color intensity is proportional to lactic acid concentrations, therefore the sample lactic acid concentration can be accurately calculated based on the lactic acid standards.
Applications :
For biological research: L-Lactate measurement in biological samples
For drug/pharm research: Drug influence on L-Lactate metabolism
Kit Components :
1. L-Lactate Assay Buffer(10x), 10ml
2. L-Lactate Standard (10x), 100ul
3. Assay Probe, 45ul
4. L-Lactate Enzyme Mix, 55ul
Detection method :
Colorimetric method at 570nm
Compatible Sample Types :
Plasma,Serum,Cell culture supernatant, other body fluid
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