Product Overview :
JMJD2D is a JmjC-domain protein that exhibits demethylation activity toward H3-K9Me3 and H3-K9Me2.
Description :
The JMJD2D Chemiluminescent Kit is designed to measure activity of JMJD2D for screening and profiling applications. The key to the JMJD2D Chemiluminescent Assay Kit is a highly specific antibody that recognizes demethylated substrate. With this kit, only three simple steps on a microtiter plate are required for methyltransferase detection. First, a sample containing JMJD2D enzyme is incubated with a sample containing assay buffer for one hour. Next, primary antibody is added. Finally, the plate is treated with an HRP-labeled secondary antibody followed by addition of the HRP substrate to produce chemiluminescence that can then be measured using a chemiluminescence reader.
Applications :
Great for studying enzyme kinetics and screening small molecular inhibitors for drug discovery and HTS applications.
Storage :
12 months from date of receipt, when stored as directed. Kit components require different storage conditions. Be sure to store each component at the proper temperature upon arrival.
Kit Components :
JMJD2D: 3 x 14 µg; -80°C Primary antibody 5: 12.5 µl; -80°C Secondary HRP-labeled antibody 1: 10 µl; -80°C 4x JMJD2D assay buffer: 3 ml; -20°C Blocking buffer: 50 ml; +4°C HRP chemiluminescent substrate A: 6 ml; +4°C HRP chemiluminescent substrate B: 6 ml; +4°C 8-well strip plate module, precoated with histone substrate: 1 (12 x 8-well strips); +4°C
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