Product Overview :
Isocitrate Assay Kit (Fluorometric) is used for quantitative determination of Isocitrate (Isocitric Acid) using fluorometric methods.
Description :
ISOCITRATE (ISOCITRIC ACID) is a substrate in the citric acid (TCA) cycle. Isocitrate is formed by the isomerization of citrate catalyzed by the enzyme aconitase. Isocitrate is oxidized by isocitrate dehydrogenase producing α-ketoglutarate and generating NADPH. Isocitrate is commonly found in many fruits and vegetables and their processed products. Industrially, isocitrate is used as a marker to identify the quality and purity of fruit juices.This isocitrate assay measures the NADPH generated from the oxidation of isocitrate. The NADPH reduces a probe into a highly fluorescent product. The fluorescence intensity of this product, measured at λ ex/em = 530/585 nm, is proportional to the isocitrate concentration in the sample.
Applications :
Isocitrate determination in food, beverage, biological samples (e.g. cell lysate, tissue homogenate, serum, etc.)
Usage :
For research use only (RUO)
Storage :
Store all components at -20°C upon receiving.
Kit Components :
Assay Buffer 10 mLNADP 500 µLStandard 1 mLEnzyme A 120 µLEnzyme B 120 µLProbe 750 µL
Detection method :
Fluorometric
Compatible Sample Types :
Beverage, Food, Cell lysate, Serum, Tissue homogenate
Features & Benefits :
Fast and sensitive. Linear detection range (20 µL sample): 0.6 to 500 µM for 10 min reaction.Convenient and high-throughput. Homogeneous "mix-incubate-measure" type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day.
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