Description :
Reactive oxygen species, such as hydrogen peroxide (H2O2) and superoxide are generated by phagocytes and participate in damaging invading miccroorganisms or other biologic targets.1 The H2O2 Cell-Based Assay Kit provides a simple fluorometric method for the sensitive quantitation of extracellular H2O2 produced by cultured cells. H2O2 is detected using ADHP (10-acetyl-3,7-dihydroxyphenoxazine), a highly sensitive and stable probe for H2O2.2 In the presence of horseradish peroxidase, ADHP reacts with H2O2 with a 1:1 stoichiometry to produce highly fluorescent resorufin.3 Resorufin fluorescence can be read using an excitation wavelength between 530-560 nm and an emission wavelength of 590 nm. Catalase, an H2O2 scavenger, is included in the kit for checking specificity of the assay.
Kit Components :
Cell-Based Assay Buffer Tablet: 1 tablet; 1 tablet; RT Cell-Based Assay Hydrogen Peroxide Standard: 1 vial/100 μl; 1 vial/100 μl; 4°C Cell-Based Assay Hydrogen Peroxide Detector ADHP: 1 vial/100 μl; 1 vial/500 μl; -20°CCell-Based Assay Horseradish Peroxidase: 1 vial/100 μl; 1 vial/500 μl; -20°CCell-Based Assay Catalase: 1 vial/2 mg; 1 vial/10 mg; -20°C
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